MPTP-Treated Zebrafish Recapitulate ‘Late-Stage’ Parkinson’s-like Cognitive Decline

В журнале Toxics,  (IF 4,146) опубликована статья с участием сотрудников Института  -

к.х.н. О.В. Ардашова (снс, ЛФАВ), д.х.н., проф. РАН К.П. Волчо (гнс, ЛФАВ)  и чл.-корр. РАН, д.х.н., проф. Н.Ф. Салахутдинова (зав. отделом медицинской химии)

MPTP-Treated Zebrafish Recapitulate ‘Late-Stage’ Parkinson’s-like Cognitive Decline

Alim A. O. Bashirzade, Sergey V. Cheresiz, Alisa S. Belova, Alexey V. Drobkov, Anastasiia D. Korotaeva, Soheil Azizi-Arani, Amirhossein Azimirad, Eric Odle, Emma-Yanina V. Gild, Oleg V. Ardashov, Konstantin P. Volcho, Dmitrii V. Bozhko, Vladislav O. Myrov, Sofia M. Kolchanova, Aleksander I. Polovian, Georgii K. Galumov, Nariman F. Salakhutdinov, Tamara G. Amstislavskaya and Allan V. Kalueff

 
(This article belongs to the Special Issue Fish Models for Human Toxicology)

Abstract

The zebrafish is a promising model species in biomedical research, including neurotoxicology and neuroactive drug screening. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) evokes degeneration of dopaminergic neurons and is commonly used to model Parkinson’s disease (PD) in laboratory animals, including zebrafish. However, cognitive phenotypes in MPTP-evoked experimental PD models remain poorly understood. Here, we established an LD₅₀ (292 mg/kg) for intraperitoneal MPTP administration in adult zebrafish, and report impaired spatial working memory (poorer spontaneous alternation in the Y-maze) in a PD model utilizing fish treated with 200 µg of this agent. In addition to conventional behavioral analyses, we also employed artificial intelligence (AI)-based approaches to independently and without bias characterize MPTP effects on zebrafish behavior during the Y-maze test. These analyses yielded a distinct cluster for 200-μg MPTP (vs. other) groups, suggesting that high-dose MPTP produced distinct, computationally detectable patterns of zebrafish swimming. Collectively, these findings support MPTP treatment in adult zebrafish as a late-stage experimental PD model with overt cognitive phenotypes. View Full-Text

Keywords: 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP); zebrafish; Parkinson’s disease; inhibitory avoidance task; spontaneous alternation; artificial intelligence

 

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