Methanethiosulfonate Derivative of OX063 Trityl: a Promising and Efficient Reagent for SDSL of Proteins

The journal Chemistry - A European Journal   (IF=5,16) published the article with the participation of NIOCh's researcher V. Tormyshev, D. Trykhin, O. Rogozhnikova, A. Spitsina, A. Kuzhelev and E/G/ Bagryanskaya

A Methanethiosulfonate Derivative of OX063 Trityl: a Promising and Efficient Reagent for SDSL of Proteins

Chemistry - A European Journal, 2020, V. 26, N 12, Pp 2705-2712
First published 18 December 2019

Abstract

Trityl radicals or TAMs have appeared recently as an alternative source of spin labels for measuring long distances in biological systems. Finland trityl radical (FTAM) served as the basis for this new generation of spin labels, but FTAM is rather lipophilic and is susceptible  to self‐aggregation, to non‐covalent binding with lipophilic sites of proteins, and to non‐covalent docking at the termini of duplex DNA. In this paper we use the very hydrophilic OX063 TAM with very‐low toxicity and  little tendency for aggregation as the basis for a spin label. Human serum albumin (HSA) labeled with OX063 has an intense, narrow line typical of TAM radicals in solution while HSA labeled with FTAM has broad lines and extensive aggregation . In pulse EPR measurements, the measured T M for HSA labelled with OX063 is 6.3 μs at 50 K, the longest yet obtained with a TAM‐based spin‐label. The lowered lipophilicity also decreases side products in the labelling reaction.

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