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N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry
Siberian Branch of Russian Academy of Science

The paper with the participation of NIOCh's researcher Alexei Yu. Vorobev is published in the journal  Biomedical Optics Express (IF 3,91

Optical uncaging of ADP reveals the early calcium dynamics in single, freely moving platelets

Darya V. Spiryova, Alexei Yu. Vorobev, Vadim V. Klimontov, Elena A. Koroleva, and Alexander E. Moskalensky

Biomedical Optics Express Vol. 11, Issue 6, pp. 3319-3330 (2020)

Published: May 27, 2020


 https://doi.org/10.1364/BOE.392745

2020_06_01_obe_Vorobev_250

 

Abstract

Platelet activation is considered to be a cornerstone in pathogenesis of cardiovascular disease. The assessment of platelet activation at the single-cell level is a promising approach for the research of platelet function in physiological and pathological conditions. Previous studies used the immobilization of platelets on the surface, which significantly alters the activation signaling. Here we show that the use of photolabile “caged” analog of ADP allows one to track the very early stage of platelet activation in single, freely moving cells. In this approach, the diffusion step and ADP receptor ligation are separated in time, and a millisecond-timescale optical pulse may trigger the activation. The technique allows us to measure the delay (lag time) between the stimulus and calcium response in platelets. We also propose a simple model function for calcium peaks, which is in good agreement with the measured data. The proposed technique and model function can be used for in-depth studies of platelet physiology.

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